Method of controlling pest

ABSTRACT

Provided is a method which exerts an excellent effect in controlling a pest in a field of soybean, corn or cotton, using a SDHI compound and a PPO-inhibiting compound. A method of controlling a pest (a weed and/or a plant pathogen) in a field of soybean, corn or a cotton, including treating a field before, at or after seeding with a seed of soybean, corn or cotton treated with one or more compounds selected from the group consisting of SDHI compounds, with one or more PPO-inhibiting compounds selected from the group consisting of flumioxazin, sulfentrazone, saflufenacil, oxyfluorfen, fomesafen and a compound of the formula:

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates to a′ method of controlling a pest,namely, a plant pathogen and a weed.

2. Description of the Related Art

SDHI compounds have been known as active ingredients of fungicides.PPO-inhibiting compounds have been known as active ingredients ofherbicides.

PRIOR ART LITERATURE Patent Literature

-   Patent Literature 1: WO 02/066471

Non-Patent Literatures

-   Non-Patent Literature 1: Crop Protection Handbook, vol. 96 (2010)-   Non-Patent Literature 2: Compendium of Pesticide Common Names    (http://www.alanwood.net/pesticides/)

SUMMARY OF THE INVENTION

The present invention provides a method which exerts excellent effectsin controlling a pest in a field of soybean, corn or cotton.

The present invention relates to the followings.

[1] A method of controlling a weed in a field of soybean, corn orcotton, comprising applying one or more PPO-inhibiting compoundsselected from the group consisting of flumioxazin, sulfentrazone,saflufenacil, oxyfluorfen, fomesafen and a compound of the formula:

to a field before, at or after seeding with seeds of soybean, corn orcotton treated with one or more compounds selected from the groupconsisting of SDHI compounds.

[2] A method of controlling a pest in a field of soybean, corn orcotton, comprising steps of:

treating a seed of soybean, corn or cotton with one of more SDHIcompounds, and

treating a field before, at or after seeding with the soybean seed, thecorn seed or the cotton seed treated with the SDHI compound, with one ormore PPO-inhibiting compounds selected from the group consisting offlumioxazin, sulfentrazone, saflufenacil, oxyfluorfen, fomesafen and acompound of the formula:

[3] The method of controlling a pest according to [1] or [2], whereinthe SDHI compound is selected from the group consisting of sedaxane,penflufen, carboxin, boscalid, furametpyr, flutolanil, fluxapyroxad,isopyrazam, fluopyram and thifluzamide.

[4] The method of controlling a pest according to [1] or [2], whereinthe SDHI compound is sedaxane, penflufen or carboxin.

[5] The method of controlling a pest according to [4], wherein thePPO-inhibiting compound is flumioxazin.

[6] The method of controlling a pest according to [2], comprising a stepof treating the field before seeding with the seed of soybean, corn orcotton, with the PPO-inhibiting compound.

[7] The method of controlling a pest according to [2], comprising a stepof treating the field to be seeded, with the PPO-inhibiting compoundsimultaneously at seeding with the seed of soybean, corn or cotton.

[8] The method of controlling a pest according to [2], comprising a stepof treating the field after seeding with the seed of soybean, corn orcotton, with the PPO-inhibiting compound.

[9] The method of controlling a pest according to [2], wherein the pestis a weed and/or a plant pathogen.

[10] The method of controlling a pest according to [2], wherein the pestis a weed.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

The method of controlling a pest according to the present inventionincludes steps of:

(1) treating a seed of soybean, corn or cotton with one or more SDHIcompounds, and

(2) treating a field before, at or after seeding with a seed of soybean,corn or cotton treated with one or more SDHI compounds, with one or morePPO-inhibiting compounds selected from the group consisting offlumioxazin, sulfentrazone, saflufenacil, oxyfluorfen, fomesafen and acompound of the formula:

In the present invention, the seed of soybean, corn or cotton is notlimited as far as it is a variety which is generally cultivated as acrop.

Examples of a plant of such a variety include plants to which resistanceto a PPO-inhibiting compound such as flumioxazin; a4-hydroxyphenylpyruvate dioxygenase-inhibiting compound such asisoxaflutole; an acetolactate synthase (hereinafter abbreviated as ALS)—inhibiting compound such as imazethapyr or thifensulfuron methyl; a5-enolpyruvylshikimate-3-phosphate synthase-inhibiting compound such asglyphosate; a glutamine synthase-inhibiting compound such asglufosinate; an auxin-type herbicide such as 2,4-D or dicamba; orbromoxynil has been imparted by a classical breeding method or a geneticengineering technique.

Examples of a crop to which resistance has been imparted by a classicalbreeding method include corn resistant to an imidazolinone typeALS-inhibiting herbicide such as imazethapyr, and this has already beencommercially available under a trade name of Clearfield (registeredtrademark). Examples of such a crop also include STS soybean which isresistant to a sulfonylurea-type ALS-inhibiting herbicide such asthifensulfuron methyl. Similarly, examples of a plant to whichresistance to an acetyl CoA carboxylase-inhibiting compound such astrione oxime-type or aryloxyphenoxypropionic acid-type herbicide hasbeen imparted by a classical breeding method include SR corn.

Examples of a plant to which resistance has been imparted by a geneticengineering technique include corn, soybean and cotton varieties whichare resistant to glyphosate, and they have already been commerciallyavailable under trade names of RoundupReady (registered trade mark),Agrisure (registered trademark) GT, Gly-Tol (registered trademark) andthe like. Similarly, there are corn, soybean and cotton varieties whichare resistant to glufosinate by a genetic engineering technique, andthey have already been commercially available under trade names ofLibertyLink (registered trademark) and the like. There are corn andsoybean varieties under the trade name of Optimum (registered trademark)GAT (registered trade mark), which are resistant to both of glyphosateand an ALS-inhibiting compound. Similarly, there are soybean varietieswhich are resistant to an imidazolinone-type ALS-inhibiting compound bya genetic engineering technique, and they have been developed under thename of Cultivance. Similarly, there is cotton varieties which areresistant to bromoxynil by a genetic engineering technique, and this hasalready been commercially available under the trade name of BXN(registered trademark).

A crop such as a soybean which is resistant to dicamba can be producedby introducing a dicamba degrading enzyme such as dicamba monooxygenaseisolated from Pseudomonas maltophilia into a plant (Behrens et al. 2007Science 316: 1185-1188).

By introducing a gene encoding aryloxyalkanoate dioxygenase, a cropwhich becomes resistant to a phenoxy acid-type herbicide such as 2,4-D,MCPA, dichlorprop or mecoprop, and an aryloxyphenoxypropionic acid-typeherbicide such as quizalofop, haloxyfop, fluazifop, diclofop,fenoxaprop, metamifop, cyhalofop and clodinafop can be produced (Wrightet al. 2010: Proceedings of National Academy of Science. 107 (47):20240-20245).

The crop includes, for example, a crop which has become possible tosynthesize a selective toxin known in Bacillus genus, using a geneticengineering technique.

Examples of the toxin which is expressed in such a geneticallyengineered plant include an insecticidal protein derived from Bacilluscereus or Bacillus popilliae; a δ-endotoxin such as Cry1Ab, Cry1Ac,Cry1F, Cry1Fa2, Cry2Ab, Cry3A, Cry3Bb1 or Cry9C, derived from Bacillusthuringiensis; an insecticidal protein such as VIP1, VIP2, VIP3 orVIP3A; an insecticidal protein derived from nematode; a toxin producedby an animal such as a scorpion toxin, a spider toxin, a bee toxin or aninsect-specific neurotoxin; a filamentous fungus toxin; plant lectin;agglutinin; a protease inhibitor such as a trypsin inhibitor, a serineprotease inhibitor, patatin, cystatin, and a papain inhibitor; aribosome inactivating protein (RIP) such as lysine, corn-RIP, abrin,luffin, saporin or bryodin; a steroid metabolism enzyme such as3-hydroxysteroid oxidase, ecdysteroid-UDP-glycosyltransferase, andcholesterol oxidase; an ecdysone inhibitor; HMG-CoA reductase; an ionchannel inhibitor such as a sodium channel inhibitor or a calciumchannel inhibitor; juvenile hormone esterase; a diuretic hormonereceptor; stilbene synthase; bibenzyl synthase; chitinase; glucanase;and the like.

A toxin expressed by such a genetically engineered crop includes ahybrid toxin of a δ-endotoxin protein such as Cry1Ab, Cry1Ac, Cry1F,Cry1Fa2, Cry2Ab, Cry3A, Cry3Bb1, Cry9C, Cry34Ab or Cry35Ab, and aninsecticidal protein such as VIP1, VIP2, VIP3 or VIP3A, and a partiallydeleted toxin, and a modified toxin. The hybrid toxin can be produced bya new combination of different domains of these proteins using a geneticengineering technique. As the partially deleted toxin, Cry1Ab in which apart of an amino acid sequence has been deleted is known. In themodified toxin, one or a plurality of amino acids of a natural toxin aresubstituted. Examples of these toxins and recombinant plants which cansynthesize these toxins are described in EP-A-0374753, WO 93/07278, WO95/34656, EP-A-0427529, EP-A-451878, WO 03/052073 and the like. Thetoxins contained in these recombinant plans impart resistance toColeoptera vermin, Diptera vermin and Lepidoptera vermin to a plant.

In addition, a genetically engineered plant containing one or aplurality of insecticidal vermin-resistant genes and expressing one or aplurality of toxins has already been known, and some of them arecommercially available. Examples of these genetically engineered plantsinclude YieldGard (registered trademark) (corn variety expressing Cry1Abtoxin), YieldGard Rootworm (registered trademark) (corn varietyexpressing Cry3Bb1 toxin), YieldGard Plus (registered trademark) (cornvariety expressing Cry1Ab and Cry3Bb1 toxins), Herculex I (registeredtrademark) (corn variety expressing phosphinothricin N-acetyltransferase(PAT) for imparting resistance to a Cry1Fa2 toxin and glufosinate),NatureGard (registered trademark), AGRISURE (registered trademark) CBAdvantage (Bt11 corn borer (CB) trait), and Protecta (registeredtrademark).

In addition, genetically engineered cotton containing one or a pluralityof insecticidal vermin-resistant genes and expressing one or a pluralityof toxins have already been known, and some of them are commerciallyavailable. Examples of these genetically engineered cotton includeBollGard (registered trademark) (cotton variety expressing Cry1Actoxin), BollGard (registered trademark) II (cotton variety expressingCry1Ac and Cry2Ab toxins), BollGard (registered trademark) III (cottonvariety expressing Cry1Ac, Cry2Ab and VIP3A toxins), VipCot (registeredtrademark) (cotton variety expressing VIP3A and Cry1Ab toxins),WideStrike (registered trademark) (cotton variety expressing Cry1Ac andCry1F toxins).

Examples of the plant used in the present invention also include plantsto which resistance to an aphid has been imparted, such as soybeans intowhich a Rag1 (Resistance Aphid Gene 1) gene has been introduced.

The crop also includes a crop to which the ability to produce ananti-pathogenic substance having selective action has been impartedusing a genetic engineering technique. As an example of theanti-pathogenic substance, a PR protein and the like are known (PRPs,EP-A-0392225). Such an anti-pathogenic substance and a geneticallyengineered plant producing the substance are described in EP-A-0392225,WO 95/33818, EP-A-0353191 and the like. Examples of the anti-pathogenicsubstance expressed in such a genetically engineered plant include anion channel inhibitor such as a sodium channel inhibitor or a calciumchannel inhibitor (KP1, KP4 and KP6 toxins, etc., which are produced byviruses, have been known); stilbene synthase; bibenzyl synthase;chitinase; glucanase; a PR protein; and an anti-pathogenic substancegenerated by microorganisms, such as a peptide antibiotic, an antibiotichaving a hetero ring, or a protein factor associated with resistance toplant diseases (which is called a plant disease-resistant gene and isdescribed in WO 03/000906).

The crop also includes a plant to which a useful character such as oilcake component modification or an amino acid content enhancing characterhas been imparted using a genetic engineering technique. Examplesthereof include VISTIVE (registered trademark) (low linolenic soybeanhaving a reduced linolenic content) and high-lysine (high-oil) corn(corn having an increased lysine or oil content).

Further, stack varieties are also included in which a plurality of theclassical herbicide character or herbicide-resistant gene, insecticidalvermin-resistant gene, anti-pathogenic substance production gene, and auseful character such as oil cake component modification or amino acidcontent enhancing character are combined.

The SDHI compound is also called a Complex II-inhibiting compound, andis a compound exhibiting a series of disease controlling activities,which acts on the mitochondrial electron transport system complex II offilamentous fungus to inhibit succinate dehydrogenase, to therebyinhibit respiration. Examples of the SDHI compound include sedaxane,penflufen, carboxin, boscalid, furametpyr, flutolanil, fluxapyroxad,isopyrazam, fluopyram and thifluzamide.

In the present invention, in the step of treating a seed of soybean,corn or cotton with the SDHI compound, the SDHI compound is usuallymixed with a solid carrier or a liquid carrier, formulated with optionaladdition of an auxiliary agent for formulation, such as a surfactant,then used.

The SDHI compound is applied at a dose in a range of usually 0.001 to 40g, preferably 0.01 to 10 g per 1 kg of the seed. Examples of a method ofapplying an active ingredient (i.e., SDHI compound) to a seed of a plantinclude a method of covering a seed with a preparation containing anactive ingredient; a method of immersing a seed in a preparationcontaining an active ingredient; and a method of coating a seed with acarrier containing an active ingredient.

In the present invention, there is a step of treating a field before, ator after seeding with a seed of soybean, corn or cotton treated with theSDHI compound, with one or more PPO-inhibiting compounds.

The PPO-inhibiting compound is a herbicidally active compound whichinhibits protoporphyrinogen IX oxidase (EC1.3.3.4) located on achlorophyll synthesis pathway in a plastid of a plant and, as a result,leads to withering of the plant.

The PPO-inhibiting compound in the present invention is flumioxazin,sulfentrazone, saflufenacil, oxyfluorfen, fomesafen and a compound ofthe formula:

(hereinafter, may be called compound A). In the present invention,fomesafen includes a free body and sodium salt of fomesafen.

In the step of treating the field with the PPO-inhibiting compound, thePPO-inhibiting compound is usually mixed with a solid carrier or aliquid carrier, formulated with optional addition of an auxiliary agentfor formulation, such as a surfactant, and then used.

Examples of a method of treating the field with the PPO-inhibitingcompound include a method of applying the PPO inhibitory compound to asoil of the field and a method of applying the PPO-inhibiting compoundto a weed after its emergence.

A dose of the PPO-inhibiting compound used in the step of treating afield with the PPO-inhibiting compound is usually in the range of 5 to5000 g per 10000 m². In the step of treating a field with thePPO-inhibiting compound, an adjuvant may be mixed upon treatment withthe PPO-inhibiting compound.

The seed of soybean, corn or cotton treated with the SDHI compound isseeded on a field by a conventional method. In the method of controllinga pest according to the present invention, the PPO-inhibiting compoundmay be applied before seeding with the seed of soybean, corn or cotton,may be applied simultaneously at seeding with the seed of soybean, cornor cotton or may be applied after seeding with the seed of soybean, cornor cotton.

When a field is treated with the PPO-inhibiting compound before seedingwith the soybean seed or the corn seed, the field is treated with thePPO-inhibiting compound 50 days before seeding to immediately beforeseeding, preferably 30 days before seeding to immediately beforeseeding, further preferably 20 days before seeding to immediately beforeseeding.

When a field is treated with the PPO-inhibiting compound after seedingwith the soybean seed or the corn seed, the field is treated with thePPO-inhibiting compound preferably immediately after seeding to 50 daysafter seeding, more preferably immediately after seeding to 3 days afterseeding. Examples of a specific treatment term when a field is treatedwith the PPO-inhibiting compound after seeding with the soybean seedinclude a term from preemergence to flowering of the soybean. Amongpreemergence to flowering of the soybean, preferred is a term frompreemergence to 6 compound leaves of the soybean, further preferred is aterm from preemergence to 3 Compound leaves of the soybean. A specifictreating term when a field is treated with the PPO-inhibiting compoundafter seeding with the corn seed is from preemergence to 12 leaves ofthe corn, preferably a term from preemergence to 8 leaves of the corn,further preferably a term from preemergence to 6 leaves of the corn. Inaddition, a leaf age of corn is determined by the Leaf Collar Method.

When a field is treated with the PPO-inhibiting compound before seedingwith the cotton seed, the field is treated with the PPO-inhibitingcompound 50 days before seeding to immediately before seeding,preferably 30 days before seeding to immediately before seeding, furtherpreferably 20 days before seeding to immediately before seeding.

When a field is treated with the PPO-inhibiting compound after seedingwith the cotton seed, the field is treated with the PPO-inhibitingcompound immediately after seeding to 70 days after seeding, preferably30 days after seeding to 50 days after seeding. Examples of a specifictreating term when a field is treated with the PPO-inhibiting compoundafter seeding with the cotton seed include pre-emergence to flowering ofthe cotton. Preferred is a lignification initiation term of the stembase of the cotton to a term when a lignification portion is 20 cm fromthe base.

According to the method of controlling a pest of the present invention,a pest such as a plant pathogen and/or a weed in a field of soybean,corn or cotton can be controlled.

Examples of the plant pathogen include the followings:

Cercospora kikuchii, icrosphaera diffusa, Diaporthe phaseolorum var.sojae, Septoria glycines, Cercospora sojina, Phakopsora pachyrhizi,Rhizoctonia solani, Sclerotinia sclerotiorum, Cercospora zeae-maydis,Elsinoe glycines, Ustilago maydis, Cochliobolus heterostrophus,Gloeocercospora sorghi, Cercospora gossypina, Phakopsora gossypii,Colletotrichum gossypii, Peronospora gossypina, Phyotophthora spp.,Aspergillus spp., Penicillium spp., Fusarium spp., Tricoderma spp.,Thielaviopsis spp., Rhizopus spp., Mucor spp., Corticium spp., Phomaspp., Diplodia spp., Verticillium spp., Puccinia spp., Mycosphaerellaspp.

Examples of the weed include the followings:

Urticaceae weeds: Urtica urens

Polygonaceae weeds: Polygonum convolvulus, Polygonum lapathifolium,Polygonum pensylvanicum, Polygonum persicaria, Polygonum longisetum,Polygonum aviculare, Polygonum arenastrum, Polygonum cuspidatum, Rumexjaponicus, Rumex crispus, Rumex obtusifolius, Rumex acetosa

Portulacaceae weeds: Portulaca oleracea

Caryophyllaceae weeds: Stellaria media, Cerastium holosteoides,Cerastium glomeratum, Spergula arvensis, Silene gallica

Aizoaceae weeds: Mollugo verticillata

Chenopodiaceae weeds: Chenopodium album, Chenopodium ambrosioides,Kochia scoparia, Salsola kali, Atriplex spp.

Amaranthaceae weeds: Amaranthus retroflexus, Amaranthus viridis,Amaranthus lividus, Amaranthus spinosus, Amaranthus hybridus, Amaranthuspalmeri, Amaranthus rudis, Amaranthus patulus, Amaranthus tuberculatos,Amaranthus blitoides, Amaranthus deflexus, Amaranthus quitensis,Alternanthera philoxeroides, Alternanthera sessilis, Alternantheratenella

Papaveraceae weeds: Papaver rhoeas, Argemone mexicana

Brassicaceae weeds: Raphanus raphanistrum, Raphanus sativus, Sinapisarvensis, Capsella bursa-pastoris, Brassica junceai, Brassicacampestris, Descurainia pinnata, Rorippa islandica, Rorippa sylvestris,Thlaspi arvense, Myagrum rugosum, Lepidium virginicum, Coronopus didymus

Capparaceae weeds: Cleome affinis

Fabaceae weeds: Aeschynomene indica, Aeschynomene rudis, Sesbaniaexaltata, Cassia obtusifolia, Cassia occidentalis, Desmodium tortuosum,Desmodium adscendens, Trifolium repens, Pueraria lobata, Viciaangustifolia, Indigofera hirsuta, Indigofera truxillensis, Vignasinensis

Oxalidaceae weeds: Oxalis corniculata, Oxalis strica, Oxalis oxyptera

Geraniaceae weeds: Geranium carolinense, Erodium cicutarium

Euphorbiaceae weeds: Euphorbia helioscopia, Euphorbia maculata,Euphorbia humistrata, Euphorbia esula, Euphorbia heterophylla, Euphorbiabrasiliensis, Acalypha australis, Croton glandulosus, Croton lobatus,Phyllanthus corcovadensis, Ricinus communis

Malvaceae weeds: Abutilon theophrasti, Sida rhombiforia, Sidacordifolia, Sida spinosa, Sida glaziovii, Sida santaremnensis, Hibiscustrionum, Anoda cristata, Malvastrum coromandelianum

Sterculiaceae weeds: Waltheria indica

Violaceae weeds: Viola arvensis, Viola tricolor

Cucurbitaceae weeds: Sicyos angulatus, Echinocystis lobata, Momordicacharantia

Lythraceae weeds: Lythrum salicaria

Apiaceae weeds: Hydrocotyle sibthorpioides

Sapindaceae weeds: Cardiospermum halicacabum

Primulaceae weeds: Anagallis arvensis

Asclepiadaceae weeds: Asclepias syriaca, Ampelamus albidus

Rubiaceae weeds: Galium aparine, Galium spurium var. echinospermon,Spermacoce latifolia, Richardia brasiliensis, Borreria alata

Convolvulaceae weeds: Ipomoea nil, Ipomoea hederacea, Ipomoea purpurea,Ipomoea hederacea var. integriuscula, Ipomoea lacunosa, Ipomoea triloba,Ipomoea acuminata, Ipomoea hederifolia, Ipomoea coccinea, Ipomoeaquamoclit, Ipomoea grandifolia, Ipomoea aristolochiafolia, Ipomoeacairica, Convolvulus arvensis, Calystegia hederacea, Calystegiajaponica, Merremia hedeacea, Merremia aegyptia, Merremia cissoides,Jacquemontia tamnifolia

Boraginaceae weeds: Myosotis arvensis

Lamiaceae weeds: Lamium purpureum, Lamium amplexicaule, Leonotisnepetaefolia, Hyptis suaveolens, Hyptis lophanta, Leonurus sibiricus,Stachys arvensis

Solanaceae weeds: Datura stramonium, Solanum nigrum, Solanum americanum,Solanum ptycanthum, Solanum sarrachoides, Solanum rostratum, Solanumaculeatissimum, Solanum sisymbriifolium, Solanum carolinense, Physalisangulata, Physalis subglabrata, Nicandra physaloides

Scrophulariaceae weeds: Veronica hederaefolia, Veronica persica,Veronica arvensis

Plantaginaceae weeds: Plantago asiatica

Asteraceae weeds: Xanthium pensylvanicum, Xanthium occidentale,Helianthus annuus, Matricaria chamomilla, Matricaria perforata,Chrysanthemum segetum, Matricaria matricarioides, Artemisia princeps,Artemisia vulgaris, Artemisia verlotorum, Solidago altissima, Taraxacumofficinale, Galinsoga ciliata, Galinsoga parviflora, Senecio vulgaris,Senecio brasiliensis, Senecio grisebachii, Conyza bonariensis, Conyzacanadensis, Ambrosia artemisiaefolia, Ambrosia trifida, Bidens pilosa,Bidens frondosa, Bidens subalternans, Cirsium arvense, Cirsium vulgare,Silybum marianum, Carduus nutans, Lactuca serriola, Sonchus oleraceus,Sonchus asper, Wedelia glauca, Melampodium perfoliatum, Emiliasonchifolia, Tagetes minuta, Blainvillea latifolia, Tridax procumbens,Porophyllum ruderale, Acanthospermum australe, Acanthospermum hispidum,Cardiospermum halicacabum, Ageratum conyzoides, Eupatorium perfoliatum,Eclipta alba, Erechtites hieracifolia, Gamochaeta spicata, Gnaphaliumspicatum, Jaegeria hirta, Parthenium hysterophorus, Siegesbeckiaorientalis, Soliva sessilis

Liliaceae weeds: Allium canadense, Allium vineale

Commelinaceae weeds: Commelina communis, Commelina bengharehsis,Commelina erecta

Poaceae weeds: Echinochloa crus-galli, Setaria viridis, Setaria faberi,Setaria glauca, Setaria geniculata, Digitaria ciliaris, Digitariasanguinalis, Digitaria horizontalis, Digitaria insularis, Eleusineindica, Poa annua, Alospecurus aequalis, Alopecurus myosuroides, Avenafatua, Sorghum halepense, Sorghum vulgare, Agropyron repens, Loliummultiflorum, Lolium perenne, Lolium rigidum, Bromus secalinus, Bromustectorum, Hordeum jubatum, Aegilops cylindrica, Phalaris arundinacea,Phalaris minor, Apera spica-venti, Panicum dichotomiflorum, Panicumtexanum, Panicum maximum, Brachiaria platyphylla, Brachiariaruziziensis, Brachiaria plantaginea, Brachiaria decumbens, Brachiariabrizantha, Brachiaria humidicola, Cenchrus echinatus, Cenchruspauciflorus, Eriochloa villosa, Pennisetum setosum, Chloris gayana,Eragrostis pilosa, Rhynchelitrum repens, Dactyloctenium aegyptium,Ischaemum rugosum, Oryza sativa, Paspalum notatum, Paspalum maritimum,Pennisetum clandestinum, Pennisetum setosum, Rottboellia cochinchinensis

Cyperaceae weeds: Cyperus microiria, Cyperus iria, Cyperus odoratus,Cyperus rotundus, Cyperus esculentus, Kyllinga gracillima

Equisetaceae weeds: Equisetum arvense, Equisetum palustre, and the like.

In the method of controlling a pest of the present invention, one ormore kinds of other agrochemicals can be also used in combinationsimultaneously or separately with the SDHI compound or thePPO-inhibiting compound. Examples of the other agrochemicals include aninsecticide, a miticide, a nematocide, a fungicide, a herbicide, a plantregulating agent and a safener.

Examples of the other agrochemicals include the following:

Herbicide: dicamba and a salt thereof (diglycolamine salt,dimethylammonium salt, isopropylammonium salt, potassium salt, sodiumsalt, choline salt), 2,4-D and a salt or ester thereof (butotyl ester,dimethylammonium salt, diolamine salt, ethylhexyl ester, isooctyl ester,isopropylammonium salt, sodium salt, triisopropanolamine salt, cholinesalt), 2,4-DB and a salt or ester thereof (dimethylammonium salt,isooctyl ester, choline salt), MCPA and a salt or ester thereof(dimethylammonium salt, 2-ethylhexyl ester, isooctyl ester, sodium salt,choline salt), MCPB, mecoprop and a salt or ester thereof(dimethylammonium salt, diolamine salt, ethadyl ester, 2-ethylhexylester, isooctyl ester, methyl ester, potassium salt, sodium salt,trolamine salt, choline salt), mecoprop-P and a salt or ester thereof(dimethylammonium salt, 2-ethylhexyl ester, isobutyl salt, potassiumsalt, choline salt), dichlorprop and a salt or ester thereof (butotylester, dimethylammonium salt, 2-ethylhexyl ester, isooctyl ester, methylester, potassium salt, sodium. salt, choline salt), dichlorprop-P,dichlorprop-P-dimethylammonium, bromoxynil, bromoxynil-octanoate,dichlobenil, ioxynil, ioxynil-octanoate, di-allate, butylate,tri-allate, phenmedipham, chlorpropham, asulam, phenisopham,benthiocarb, molinate, esprocarb, pyributicarb, prosulfocarb, orbencarb,EPTC, dimepiperate, swep, propachlor, metazachlor, alachlor, acetochlor,metolachlor, S-metolachlor, butachlor, pretilachlor, thenylchlor,aminocyclopyrachlor, aminocyclopyrachlor-methyl,aminocyclopyrachlor-potassium, trifluralin, pendimethalin,ethalfluralin, benfluralin, prodiamine, simazine, atrazine, propazine,cyanazine, ametryn, simetryn, dimethametryn, prometryn, indaziflam,triaziflam, metribuzin, hexazinone, isoxaben, diflufenican, diuron,linuron, fluometuron, difenoxuron, methyl-daimuron, isoproturon,isouron, tebuthiuron, benzthiazuron, methabenzthiazuron, propanil,mefenacet, clomeprop, naproanilide, bromobutide, daimuron, cumyluron,diflufenzopyr, etobenzanid, bentazon, tridiphane, indanofan, amitrole,fenchlorazole, clomazone, maleic hydrazide, pyridate, chloridazon,norflurazon, bromacil, terbacil, oxaziclomefone, cinmethylin,benfuresate, cafenstrole, pyrithiobac, pyrithiobac-sodium, pyriminobac,pyriminobac-methyl, bispyribac, bispyribac-sodium, pyribenzoxim,pyrimisulfan, pyriftalid, fentrazamide, dimethenamid, dimethenamid-P,ACN, bennzobicyclon, dithiopyr, triclopyr and a salt or ester thereof(butotyl ester, triethylammonium salt), fluoroxypyr, fluoroxypyr-meptyl,thiazopyr, aminopyralid and a salt thereof (potassium salt,triisopropanolammonium salt, choline salt), clopyralid and a saltthereof (olamine salt, potassium salt, triethylammonium salt, cholinesalt), picloram and a salt thereof (potassium salt,triisopropanolammonium salt, choline salt), dalapon, chlorthiamid,amidosulfuron, azimsulfuron, bensulfuron, bensulfuron-methyl,chlorimuron, chlorimuron-ethyl, cyclosulfamuron, ethoxysulfuron,flazasulfuron, flucetosulfuron, flupyrsulfuron,flupyrsulfuron-methyl-sodium, foramsulfuron, halosulfuron,halosulfuron-methyl, imazosulfuron, mesosulfuron, mesosulfuron-methyl,nicosulfuron, orthosulfamuron, oxasulfuron, primisulfuron,primisulfuron-methyl, propyrisulfuron, pyrazosulfuron,pyrazosulfuron-ethyl, rimsulfuron, sulfometuron, sulfometuron-methyl,sulfosulfuron, trifloxysulfuron-sodium, trifloxysulfuron, chlorsulfuron,cinosulfuron, ethametsulfuron, ethametsulfuron-methyl, iodosulfuron,iodosulfuron-methyl-sodium, metsulfuron, metsulfuron-methyl,prosulfuron, thifensulfuron, thifensulfuron-methyl, triasulfuron,tribenuron, tribenuron-methyl, triflusulfuron, triflusulfuron-methyl,tritosulfuron, picolinafen, beflubutamid, mesotrione, sulcotrione,tefuryltrione, tembotrione, isoxachlortole, isoxaflutole, benzofenap,pyrasulfotole, pyrazolynate, pyrazoxyfen, topramezone, flupoxam,amicarbazone, bencarbazone, flucarbazone, flucarbazone-sodium,ipfencarbazone, propoxycarbazone, propoxycarbazone-sodium,thiencarbazone, thiencarbazone-methyl, cloransulam, cloransulam-methyl,diclosulam, florasulam, flumetsulam, metosulam, penoxsulam, pyroxsulam,imazamethabenz, imazamethabenz-methyl, imazamox, imazamox-ammonium,imazapic, imazapic-ammonium, imazapyr, imazapyr-ammonium, imazaquin,imazaquin-ammonium, imazethapyr, imazethapyr-ammonium, clodinafop,clodinafop-propargyl, cyhalofop, cyhalofop-butyl, diclofop,diclofop-methyl, fenoxaprop, fenoxaprop-ethyl, fenoxaprop-P,fenoxaprop-P-ethyl, fluazifop, fluazifop-butyl, fluazifop-P,fluazifop-P-butyl, haloxyfop, haloxyfop-methyl, haloxyfop-P,haloxyfop-P-methyl, metamifop, propaquizafop, quizalofop,quizalofop-ethyl, quizalofop-P, quizalofop-P-ethyl, alloxydim,clethodim, sethoxydim, tepraloxydim, tralkoxydim, pinoxaden,pyroxasulfone, glyphosate, glyphosate-isopropylamine,glyphosate-trimethylsulfonium, glyphosate-ammonium,glyphosate-diammonium, glyphosate-sodium, glyphosate-potassium,glyphosate-guanidine, glufosinate, glufosinate-ammonium, glufosinate-P,glufosinate-P-sodium, bialafos, anilofos, bensulide, butamifos,paraquat, paraquat-dichloride, diquat and diquat-dibromide

Plant growth regulating agents: hymexazol, paclobutrazol, uniconazole,uniconazole-P, inabenfide, prohexadione-calcium, 1-methylcyclopropene,trinexapac and gibberellins.

Safeners: benoxacor, cloquintocet, cloquintocet-mexyl, cyometrinil,cyprosulfamide, dichlormid, dicyclonon, dietholate, fenchlorazole,fenchlorazole-ethyl, fenclorim, flurazole, fluxofenim, furilazole,isoxadifen, isoxadifen-ethyl, mefenpyr, mefenpyr-diethyl, mephenate,naphthalic anhydride and oxabetrinil.

EXAMPLES

The present invention will be described below by way of examples, butthe present invention is not limited to these examples.

First, evaluation criteria of herbicidal activity and phytotoxicity oncrop will be shown.

Evaluation of herbicidal activity is classified into 0 to 100, lettingno or little difference when the state of germination or growth of atest weed at investigation is compared with that of non-treatment to be“0”, and letting complete withering of a test weed or completeinhibition of germination or growth to be “100”.

For evaluation of phytotoxicity on crop, when phytotoxicity is hardlyperceived, it is represented by “no damage”, when slight phytotoxicityis perceived, it is represented by “slight”, when moderate phytotoxicityis perceived, it is represented by “moderate”, and when severephytotoxicity is perceived, it is represented by “severe”.

Example 1 Pre-Plant Application in Cotton

In combinations shown in Table 1, herbicidal-activity against a weed andphytotoxicity on a crop can be confirmed according to the aforementionedcriteria, by the following method.

A soil is packed into a pot, a weed is seeded, and the soil surface isuniformly treated with a PPO-inhibiting compound. After 15 days, cottonseeds with a SDHI compound attached thereto are seeded. This pot isplaced in a greenhouse. Fifteen days after seeding, the herbicidalactivity against the weed is investigated.

TABLE 1 Combination SDHI compound PPO-inhibiting compound 1-1 SedaxaneFlumioxazin 1-2 Penflufen Flumioxazin 1-3 Carboxin Flumioxazin 1-4Sedaxane Fomesafen 1-5 Penflufen Fomesafen 1-6 Carboxin Fomesafen 1-7Sedaxane Oxyfluorfen 1-8 Penflufen Oxyfluorfen 1-9 Carboxin Oxyfluorfen1-10 Sedaxane Saflufenacil 1-11 Penflufen Saflufenacil 1-12 CarboxinSaflufenacil 1-13 Sedaxane Compound A 1-14 Penflufen Compound A 1-15Carboxin Compound A

Example 2 Post-Directed Application in Cotton

In combinations shown in Table 2, herbicidal activity against a weed andphytotoxicity on a crop can be confirmed according to the aforementionedcriteria, by the following method.

A SDHI compound is attached to each of cotton seeds. Then, the seeds areseeded on a cultivated land. Thirty days after seeding, in the statewhere the main stem of cotton is lignified 15 cm from the groundsurface, the cultivated land is subjected to Post-directed applicationwith a PPO-inhibiting compound. Twenty eight days after the treatment,the herbicidal activity against the weed is investigated.

TABLE 2 Combination SDHI compound PPO-inhibiting compound 2-1 SedaxaneFlumioxazin 2-2 Penflufen Flumioxazin 2-3 Carboxin Flumioxazin 2-4Sedaxane Fomesafen 2-5 Penflufen Fomesafen 2-6 Carboxin Fomesafen 2-7Sedaxane Oxyfluorfen 2-8 Penflufen Oxyfluorfen 2-9 Carboxin Oxyfluorfen2-10 Sedaxane Saflufenacil 2-11 Penflufen Saflufenacil 2-12 CarboxinSaflufenacil 2-13 Sedaxane Compound A 2-14 Penflufen Compound A 2-15Carboxin Compound A

Example 3 Pre-Plant Application in Soybean

In combinations shown in Table 3, a controlling effect on a weed andphytotoxicity on a crop can be confirmed according to the aforementionedcriteria, by the following method.

A soil is packed into a pot, a weed is seeded, and a soil surface isuniformly treated with a PPO-inhibiting compound. After 7 days, soybeanseeds with a SDHI compound attached thereto are seeded. This pot isplaced in a greenhouse. Fifteen days after seeding, the herbicidalactivity against the weed is investigated.

TABLE 3 Combination SDHI compound PPO-inhibiting compound 3-1 SedaxaneFlumioxazin 3-2 Penflufen Flumioxazin 3-3 Carboxin Flumioxazin 3-4Sedaxane Fomesafen 3-5 Penflufen Fomesafen 3-6 Carboxin Fomesafen 3-7Sedaxane Saflufenacil 3-8 Penflufen Saflufenacil 3-9 CarboxinSaflufenacil 3-10 Sedaxane Oxyfluorfen 3-11 Penflufen Oxyfluorfen 3-12Carboxin Oxyfluorfen 3-13 Sedaxane Compound A 3-14 Penflufen Compound A3-15 Carboxin Compound A 3-16 Sedaxane Sulfentrazone 3-17 PenflufenSulfentrazone 3-18 Carboxin Sulfentrazone

Example 4 Preemergence Application in Soybean

In combinations shown in Table 4, herbicidal activity against a weed andphytotoxicity on a crop can be confirmed according to the aforementionedcriteria, by the following method.

A SDHI compound is attached to each of soybean seeds. Then, a soil ispacked into a pot, and the seeds and seeds of a weed are seeded. On theday of the seeding, the soil surface is uniformly treated with aPPO-inhibiting compound. This pot is placed in a greenhouse. Fifteendays after seeding, the herbicidal activity against the weed isinvestigated.

TABLE 4 Combination SDHI compound PPO-inhibiting compound 4-1 SedaxaneFlumioxazin 4-2 Penflufen Flumioxazin 4-3 Carboxin Flumioxazin 4-4Sedaxane Fomesafen 4-5 Penflufen Fomesafen 4-6 Carboxin Fomesafen 4-7Sedaxane Saflufenacil 4-8 Penflufen Saflufenacil 4-9 CarboxinSaflufenacil 4-10 Sedaxane Compound A 4-11 Penflufen Compound A 4-12Carboxin Compound A 4-13 Sedaxane Sulfentrazone 4-14 PenflufenSulfentrazone 4-15 Carboxin Sulfentrazone

Example 5 Preemergence Application in Corn

In combinations shown in Table 5, herbicidal activity against a weed andphytotoxicity on a crop can be confirmed according to the aforementionedcriteria, by the following method.

A SDHI compound is attached to each of corn seeds. Then, a soil ispacked into a pot, and the seeds and seeds of a weed are seeded. On theday of the seeding, the soil surface is uniformly treated with aPPO-inhibiting compound. This pot is placed in a greenhouse. Fifteendays after the seeding, the herbicidal activity against the weed isinvestigated.

TABLE 5 Combination SDHI compound PPO-inhibiting compound 5-1 SedaxaneSaflufenacil 5-2 Penflufen Saflufenacil 5-3 Carboxin Saflufenacil 5-4Sedaxane Compound A 5-5 Penflufen Compound A 5-6 Carboxin Compound A

Example 6 Pre-Plant Application in Corn

In combinations shown in Table 6, herbicidal activity against a weed andphytotoxicity on a crop can be confirmed according to the aforementionedcriteria, by the following method.

A soil is packed in a pot, a weed is seeded, and the soil surface isuniformly treated with a PPO-inhibiting compound. After 7 days, cornseeds with a SDHI compound attached thereto are seeded. This pot isplaced in a greenhouse. Fifteen days after the seeding, the herbicidalactivity against the weed is investigated.

TABLE 6 Combination SDHI compound PPO-inhibiting compound 6-1 SedaxaneFlumioxazin 6-2 Penflufen Flumioxazin 6-3 Carboxin Flumioxazin 6-4Sedaxane Saflufenacil 6-5 Penflufen Saflufenacil 6-6 CarboxinSaflufenacil 6-7 Sedaxane Compound A 6-8 Penflufen Compound A 6-9Carboxin Compound A

According to the method of controlling a pest according to the presentinvention, a pest in a field of soybean, corn or cotton can beeffectively controlled.

What is claimed is:
 1. A method of controlling a weed in a field ofsoybean, corn or cotton, comprising applying one or more PPO-inhibitingcompounds selected from the group consisting of flumioxazin,sulfentrazone, saflufenacil, oxyfluorfen, fomesafen and a compound ofthe formula:

to a field before, at or after seeding with a seed of soybean, corn orcotton treated one or more compounds selected from the group consistingof SDHI compounds.
 2. A method of controlling a pest in a field ofsoybean, corn or cotton, comprising steps of: treating a seed ofsoybean, corn or cotton with one or more compounds selected from SDHIcompounds, and treating a field before, at or after seeding with theseed of soybean, corn or cotton treated with the SDHI compound, with oneor more PPO-inhibiting compounds selected from the group consisting offlumioxazin, sulfentrazone, saflufenacil, oxyfluorfen, fomesafen and acompound of the formula:


3. The method of controlling a pest according to claim 1 or 2, whereinthe SDHI compound is selected from the group consisting of sedaxane,penflufen, carboxin, boscalid, furametpyr, flutolanil, fluxapyroxad,isopyrazam, fluopyram and thifluzamide.
 4. The method of controlling apest according to claim 1 or 2, wherein the SDHI compound is sedaxane,penflufen or carboxin.
 5. The method of controlling a pest according toclaim 4, wherein the PPO-inhibiting compound is flumioxazin.
 6. Themethod of controlling a pest according to claim 2, comprising a step oftreating the field before seeding with the seed of soybean, corn orcotton, with the PPO inhibitory compound.
 7. The method of controlling apest according to claim 2, comprising a step of treating the field to beseeded, with the PPO-inhibiting compound simultaneously at seeding withthe seed of soybean, corn or cotton.
 8. The method of controlling a pestaccording to claim 2, comprising a step of treating the field afterseeding with the seed of soybean, corn or cotton, with thePPO-inhibiting compound.
 9. The method of controlling a pest accordingto claim 2, wherein the pest is a weed and/or a plant pathogen.
 10. Themethod of controlling a pest according to claim 2, wherein the pest is aweed.